SYK [p Tyr526, p Tyr525] Antibody (PSH03-70)

Immunocytochemistry/ Immunofluorescence: SYK [p Tyr526, p Tyr525] Antibody (PSH03-70) [NBP3-32800]

Immunocytochemistry/ Immunofluorescence: SYK [p Tyr526, p Tyr525] Antibody (PSH03-70) [NBP3-32800] - Immunocytochemistry analysis of Ramos cells serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes labeling SYK with Rabbit anti-SYK antibody (NBP3-32800) at 1/1,000 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SYK antibody (NBP3-32800) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Western Blot: SYK [p Tyr526, p Tyr525] Antibody (PSH03-70) [NBP3-32800]

Western Blot: SYK [p Tyr526, p Tyr525] Antibody (PSH03-70) [NBP3-32800] - Western blot analysis of SYK on different lysates with Rabbit anti-SYK antibody (NBP3-32800) at 1/1,000 dilution.
Lane 1: Ramos cell lysate
Lane 2: Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 72 kDa
Observed band size: 72 kDa

Exposure time:
Lane 1-2 (left): 1 minute 2 seconds;
Lane 1-2 (right): 2 minutes 18 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.