SYK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1796

Novus Biologicals, part of Bio-Techne
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NB100-1796
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all SYK Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KYLQQNRHVKDKN corresponding to internal region according to NP_001128524.1.

Specificity

This antibody is expected to recognise both reported isoforms (NP_003168.2; NP_001128524.1). Reported pairs of variants represent identical proteins (NP_003168.2 and NP_001167638.1; NP_001128524.1 and NP_001167639.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SYK Antibody

Western Blot: SYK Antibody [NB100-1796]

Western Blot: SYK Antibody [NB100-1796]

Western Blot: SYK Antibody [NB100-1796] - Analysis of SYK in Human Spleen lysate using this antibody at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for SYK Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in human bone marrow, lymph node, and spleen lysates (calculated MW of 72.1 kDa band according to NP_003168.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SYK

Syk (72 kDa) is a non-receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.

Long Name

Spleen Tyrosine Kinase

Alternate Names

DKFZp313N1010, EC 2.7.10, EC 2.7.10.2, FLJ25043, spleen tyrosine kinaseFLJ37489, tyrosine-protein kinase SYK

Entrez Gene IDs

6850 (Human); 20963 (Mouse); 25155 (Rat)

Gene Symbol

SYK

UniProt

NP_003168.2 (Human)

Additional SYK Products

Product Documents for SYK Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SYK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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