Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Symplekin Antibodies »

Product Summary for Symplekin Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 350 and 400 of human Symplekin using the numbering given in entry NP_004810.2 (GeneID 8189).

Localization

Cytoplasm, cytoskeleton. Cell junction, adherens junction; Cytoplasmic side. Cell junction; Cytoplasmic side. Nucleus, nucleoplasm. Note=Cytoplasmic face of adhesion plaques (major) and nucleoplasm (minor) (in cells with TJ). Nucleoplasm (in cells without

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

141 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Symplekin Antibody

Western Blot: Symplekin Antibody [NB100-74591]

Western Blot: Symplekin Antibody [NB100-74591]

Western Blot: Symplekin Antibody [NB100-74591] - Detection of Human and Mouse Symplekin by Western Blot. Samples: Whole cell lysate from HeLa, (5, 15 and 50 ug), and 293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Symplekin antibody NB100-74591 used for WB at 0.02 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Symplekin Antibody [NB100-74591]

Immunoprecipitation: Symplekin Antibody [NB100-74591]

Immunoprecipitation: Symplekin Antibody [NB100-74591] - Detection of human Symplekin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Symplekin antibody NB100-74591 (lot 2) used for IP at 6 ug per reaction. Symplekin was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-Symplekin antibody NB100-74590. For blotting immunoprecipitated Symplekin, NB100-74591 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Symplekin Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Symplekin

This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.

Alternate Names

FLJ27092, SPKSYM, symplekin

Entrez Gene IDs

8189 (Human)

Gene Symbol

SYMPK

UniProt

Q92797 (Human)

Additional Symplekin Products

Product Documents for Symplekin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Symplekin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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