Symplekin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92464

Novus Biologicals, part of Bio-Techne
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NBP1-92464
NBP1-92464-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Symplekin Antibodies »

Product Summary for Symplekin Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PNPPSVLFGADKDTEVAAPWTEETVKQCLYLYLALLPQNHKLIHELAAVYTEAIADIKRTVLRVIEQPIRGMGMNSPELLLLVENCPKGA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Symplekin Antibody

Immunocytochemistry/ Immunofluorescence: Symplekin Antibody [NBP1-92464]

Immunocytochemistry/ Immunofluorescence: Symplekin Antibody [NBP1-92464]

Immunocytochemistry/Immunofluorescence: Symplekin Antibody [NBP1-92464] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.
Immunohistochemistry-Paraffin: Symplekin Antibody [NBP1-92464]

Immunohistochemistry-Paraffin: Symplekin Antibody [NBP1-92464]

Immunohistochemistry-Paraffin: Symplekin Antibody [NBP1-92464] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.

Applications for Symplekin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Symplekin

Symplekin encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.

Alternate Names

FLJ27092, SPKSYM, symplekin

Gene Symbol

SYMPK

Additional Symplekin Products

Product Documents for Symplekin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Symplekin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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