Synapsin I [p Ser9] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-180

Novus Biologicals, part of Bio-Techne
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NB300-180
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat, Zebrafish

Predicted:

Bovine (100%), Human (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Synapsin I Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 conjugated to KLH. Accession # P17599

Modification

p Ser9

Localization

Cell junction; synapse

Specificity

Specific for endogenous levels of the ~78 kDa synapsin I doublet protein phosphorylated at Ser9. Also weakly labels the ~55 kDa synapsin II protein which has a similar phosphorylation site to that of Ser9 on synapsin I. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Marker

pre-Synaptic Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Synapsin I [p Ser9] Antibody

Western Blot: Synapsin I [p Ser9] Antibody [NB300-180]

Western Blot: Synapsin I [p Ser9] Antibody [NB300-180]

Western Blot: Synapsin I [p Ser9] Antibody [NB300-180] - Rat cortex lysate showing specific immunolabeling of the ~78k synapsin I phosphorylated at Ser9 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser9 synapsin I antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
Immunohistochemistry: Synapsin I [p Ser9] Antibody [NB300-180]

Immunohistochemistry: Synapsin I [p Ser9] Antibody [NB300-180]

Immunohistochemistry: Synapsin I [p Ser9] Antibody [NB300-180] - Cultured mouse caudate neurons showing synapsin I when phosphorylated at Ser9

Applications for Synapsin I [p Ser9] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:500

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Synapsin I

Synapsin I (synapsin Ia and Ib, MW ~ 80,000 and 77,000 are collectively referred to as synapsin I) is a neuron specific protein that is localized to nerve terminals. The synapsin protein is an excellent marker for synaptic terminals and it can be used to estimate synaptic density and or synaptogenesis. It is thought that synapsin I cross-links synaptic vesicles to the cytoskeleton and thereby limits the ability of these synaptic vesicles to move to active zones and fuse with the plasma membrane during exocytosis. In addition to their role in neurotransmission, the synapsins are also thought to play a role in synapse formation. The appearance of synapsin I immunoreactivity correlates precisely with the development of synapses in the CNS. The synapsin protein is an excellent marker for synaptic terminals and it can be used to estimate synaptic density and or synaptogenesis. The appearance of synapsin I was a precise indicator of synapse formation and that synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis.

Alternate Names

SYN1, phospho Synapsin

Entrez Gene IDs

20964 (Mouse); 24949 (Rat)

Gene Symbol

SYN1

UniProt

P09951 (Rat)

Additional Synapsin I Products

Product Documents for Synapsin I [p Ser9] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Synapsin I [p Ser9] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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