Synapsin I Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS062

R&D Systems, part of Bio-Techne
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PPS062
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

View all Synapsin I Antibodies »

Product Specifications

Immunogen

Synapsin I

Specificity

Human, rat, mouse, and bovine ~85 kDa Synapsin I in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Scientific Data Images for Synapsin I Antibody

Detection of Synapsin I antibody by Western Blot.

Detection of Synapsin I by Western Blot.

Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 78 kDa Synapsin I doublet protein. Immunolabeling blocked by preadsorption of the antibody with the protein used to generate the antibody (not shown).

Applications for Synapsin I Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution

Formulation, Preparation, and Storage

Purification

N/A

Formulation

50 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: Synapsin I

Synapsin I is an 85 kDa neuron-specific, O-GlcNAcylated phosphoprotein that interacts with synaptic vesicles and regulates neurotransmitter release. It is concentrated in presynaptic terminals where it anchors synaptic vesicles to the underlying cytoskeleton. Phosphorylation at various sites reduces synapsin I binding to neurosecretory vesicles, making them available for neurotransmitter release. Rat synapsin I is 704 amino acids (aa) in length and contains five domains, named
A-E/F. Domains A-D lie between aa 1-655, while E and F are alternate splice forms. Domain A (28 aa) regulates neurotransmitter release in a phosphorylation-dependent manner. Domains C (328 aa) and E (49 aa) anchor to actin in the synaptic region. Domains B (84 aa) and D (235 aa) promote actin and secretory vesicle interaction, and actin nucleation. Phosphorylation of Ser603 and 566 in domain D, and Ser9 in domain A, reduces the affinity of synapsin I for actin and secretory vesicles.

Alternate Names

SYN1

Entrez Gene IDs

6853 (Human); 20964 (Mouse); 24949 (Rat)

Gene Symbol

SYN1

Additional Synapsin I Products

Product Documents for Synapsin I Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Synapsin I Antibody

  1. Yamagata, Y. (2003) J. Pharmacol. Sci. 93:22.
  2. Sakurada, K. et al. (2002) J. Biol. Chem. 277:45473.
  3. Hilfiker, S. et al. (2005) J. Neurosci. 25:2658.
  4. McCaffery, C.A. and L.J. DeGennaro (1986) EMBO J. 5:3167.
  5. Cole, R.N. and G.W. Hart (1999) J. Neurochem. 73:418.

For research use only

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