Synapsin II Antibody (GT1446)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13694
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # GT1446
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Synapsin II. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Synapsin II Antibody (GT1446)
Western Blot: Synapsin II Antibody (GT1446) [NBP3-13694]
Western Blot: Synapsin II Antibody (GT1446) [NBP3-13694] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody [GT1446] (NBP3-13694) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: Synapsin II Antibody (GT1446) [NBP3-13694]
Immunocytochemistry/Immunofluorescence: Synapsin II Antibody (GT1446) [NBP3-13694] - Synapsin II antibody [GT1446] detects Synapsin II protein at synapse by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Synapsin II stained by Synapsin II antibody [GT1446] (NBP3-13694) diluted at 1:500. Red: Tau, a axon marker, stained by Tau antibody diluted at 1:500.Immunohistochemistry-Paraffin: Synapsin II Antibody (GT1446) [NBP3-13694]
Immunohistochemistry-Paraffin: Synapsin II Antibody (GT1446) [NBP3-13694] - Synapsin II antibody [GT1446] detects Synapsin II protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Synapsin II stained by Synapsin II antibody [GT1446] (NBP3-13694) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for Synapsin II Antibody (GT1446)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 20% Glycerol
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Synapsin II
Alternate Names
synapsin IISYNII, synapsin-2, SYNIIa, SYNIIb
Gene Symbol
SYN2
Additional Synapsin II Products
Product Documents for Synapsin II Antibody (GT1446)
Product Specific Notices for Synapsin II Antibody (GT1446)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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