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Synaptojanin 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87842

Novus Biologicals, part of Bio-Techne
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NBP1-87842
NBP1-87842-25ul

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ASFGEVILIRFVEDKMWVTFLEGSSALNVLSLNGKELLNRTITIALKSPDWIKNLEEEMSLEKISIALPSSTSSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQS

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32356558).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Synaptojanin 1 Antibody

Knockdown Validated: Synaptojanin 1 Antibody [NBP1-87842]

Knockdown Validated: Synaptojanin 1 Antibody [NBP1-87842]

Knockdown Validated: Synaptojanin 1 Antibody [NBP1-87842] - (A) The levels of synj1 protein in N2a cells after synj1 siRNA. Results are presented as % of levels in controls +/- SEM. (B) A representative example of western blot analysis. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-11654-7) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842]

Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842]

Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding SYNJ1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Synaptojanin 1 Antibody [NBP1-87842]

Immunocytochemistry/ Immunofluorescence: Synaptojanin 1 Antibody [NBP1-87842]

Immunocytochemistry/Immunofluorescence: Synaptojanin 1 Antibody [NBP1-87842] - Staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol and centrosome. Antibody staining is shown in green.

Applications for Synaptojanin 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

Reported in scientific literature (PMID: 26372964)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Synaptojanin 1

Synaptojanin 1 [formerly known as synaptojanin] is an inositol 5-phosphatase which exists in two tissue specific isoforms (170 and 145 kDa). Synaptojanin is involved in clathrin-mediated endocytosis.

Alternate Names

EC 3.1.3, EC 3.1.3.36, KIAA0910, polyphosphoinositide phosphatase, 10synaptojanin-1, Synaptic inositol-14,5-trisphosphate 5-phosphatase 1, synaptojanin 1

Gene Symbol

SYNJ1

Additional Synaptojanin 1 Products

Product Documents for Synaptojanin 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Synaptojanin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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