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Synaptojanin 2 Antibody (2H8)

Novus Biologicals, part of Bio-Techne | Catalog # H00008871-M02

Novus Biologicals, part of Bio-Techne
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H00008871-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2H8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

SYNJ2 (NP_003889, 1396 a.a. ~ 1495 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GTKAMKPEAAPLLGDYQDPFWNLLHHPKLLNNTWLSKSSDPLDSGTRSPKRDPIDPVSAGASAAKAELPPDHEHKTLGHWVTISDQEKRTALQVFDPLAK

Localization

Membrane. Note=Predominantly associated with the particulate fractions.

Specificity

SYNJ2 - synaptojanin 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Synaptojanin 2 Antibody (2H8)

Western Blot: Synaptojanin 2 Antibody (2H8) [H00008871-M02]

Western Blot: Synaptojanin 2 Antibody (2H8) [H00008871-M02]

Western Blot: Synaptojanin 2 Antibody (2H8) [H00008871-M02] - Analysis of SYNJ2 over-expressed 293 cell line, cotransfected with SYNJ2 Validated Chimera RNAi ( Cat # H00008871-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SYNJ2 monoclonal antibody (M02), clone 2H8 (Cat # H00008871-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: Synaptojanin 2 Antibody (2H8) [H00008871-M02]

Western Blot: Synaptojanin 2 Antibody (2H8) [H00008871-M02]

Western Blot: Synaptojanin 2 Antibody (2H8) [H00008871-M02] - Analysis of SYNJ2 expression in transfected 293T cell line by SYNJ2 monoclonal antibody (M02), clone 2H8.Lane 1: SYNJ2 transfected lysate(92.2 KDa).Lane 2: Non-transfected lysate.

Applications for Synaptojanin 2 Antibody (2H8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Synaptojanin 2

Synaptojanin-2 is a ubiquitously expressed inositol polyphosphate 5-phosphatase (Malecz et al., 2000 [PubMed 11084340]).[supplied by OMIM]

Alternate Names

EC 3.1.3.36, inositol phosphate 5'-phosphatase 2, INPP5H, KIAA0348MGC44422, Synaptic inositol-14,5-trisphosphate 5-phosphatase 2, synaptojanin 2, synaptojanin-2

Entrez Gene IDs

8871 (Human)

Gene Symbol

SYNJ2

OMIM

609410 (Human)

Additional Synaptojanin 2 Products

Product Documents for Synaptojanin 2 Antibody (2H8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Synaptojanin 2 Antibody (2H8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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