Synaptophysin Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25170
![Western Blot: Synaptophysin AntibodyBSA Free [NBP2-25170]](https://resources.bio-techne.com/images/products/Synaptophysin-Antibody-Western-Blot-NBP2-25170-img0001.jpg "Western Blot: Synaptophysin AntibodyBSA Free [NBP2-25170]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Primate
Cited:
Human, Mouse, Rat
Predicted:
Bovine (96%), Rat (95%), Squirrel (97%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to an C-terminal portion of the human Synaptophysin protein (between residues 230-313) [UniProt# P08247]
Localization
Golgi, cytoplasmic and membrane
Marker
Pre-synaptic marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Synaptophysin Antibody - BSA Free
Western Blot: Synaptophysin AntibodyBSA Free [NBP2-25170]
Western Blot: Synaptophysin Antibody [NBP2-25170] - Analysis of Synaptophysin in (A) human brain lysate and (B) mouse brain lysate.![Immunocytochemistry/ Immunofluorescence: Synaptophysin Antibody - BSA Free [NBP2-25170]](https://resources.bio-techne.com/images/products/Synaptophysin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25170-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Synaptophysin Antibody - BSA Free [NBP2-25170]")
Immunocytochemistry/ Immunofluorescence: Synaptophysin Antibody - BSA Free [NBP2-25170]
Immunocytochemistry/Immunofluorescence: Synaptophysin Antibody [NBP2-25170] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Synaptophysin at a 1:50 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Immunohistochemistry: Synaptophysin Antibody - BSA Free [NBP2-25170]](https://resources.bio-techne.com/images/products/Synaptophysin-Antibody-Immunohistochemistry-NBP2-25170-img0002.jpg "Immunohistochemistry: Synaptophysin Antibody - BSA Free [NBP2-25170]")
Immunohistochemistry: Synaptophysin Antibody - BSA Free [NBP2-25170]
Immunohistochemistry: Synaptophysin Antibody [NBP2-25170] - Analysis of Synaptophysin in mouse brain.Applications for Synaptophysin Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:250
Immunohistochemistry
1:100
Immunohistochemistry-Frozen
reported in scientific literature (PMID 31117227)
Immunohistochemistry-Paraffin
1:100
Western Blot
1.0 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Synaptophysin
Alternate Names
SYP
Gene Symbol
SYP
UniProt
Additional Synaptophysin Products
Product Documents for Synaptophysin Antibody - BSA Free
Product Specific Notices for Synaptophysin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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