Synaptopodin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87887

Novus Biologicals, part of Bio-Techne
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NBP1-87887
NBP1-87887-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Synaptopodin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TTPTKVYSEVHFTLAKPPSVVNRTARPFGIQAPGGTSQMERSPMLERRHFGEKAPAPQPPSLPDRSPRPQRHIMSRSPMVERRMMGQRS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%). Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Synaptopodin Antibody

Western Blot: Synaptopodin Antibody [NBP1-87887]

Western Blot: Synaptopodin Antibody [NBP1-87887]

Western Blot: Synaptopodin Antibody [NBP1-87887] - Analysis in human skeletal muscle tissue.
Immunocytochemistry/ Immunofluorescence: Synaptopodin Antibody [NBP1-87887]

Immunocytochemistry/ Immunofluorescence: Synaptopodin Antibody [NBP1-87887]

Immunocytochemistry/Immunofluorescence: Synaptopodin Antibody [NBP1-87887] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Synaptopodin Antibody [NBP1-87887]

Immunohistochemistry-Paraffin: Synaptopodin Antibody [NBP1-87887]

Immunohistochemistry-Paraffin: Synaptopodin Antibody [NBP1-87887] - Staining of human tonsil shows no positivity in lymphoid cells as expected.
View 3 more images

Applications for Synaptopodin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Synaptopodin

Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).

Alternate Names

SYNPO

Gene Symbol

SYNPO

Additional Synaptopodin Products

Product Documents for Synaptopodin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Synaptopodin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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