Synaptotagmin 12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12546

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Synaptotagmin 12 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human Synaptotagmin 12 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for Synaptotagmin 12 Antibody

Western Blot: Synaptotagmin 12 Antibody [NBP3-12546]

Western Blot: Synaptotagmin 12 Antibody [NBP3-12546]

Western Blot: Synaptotagmin 12 Antibody [NBP3-12546] - Lane A: COS7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:46 kDaObserved band size:46 kDa
Immunohistochemistry-Paraffin: Synaptotagmin 12 Antibody [NBP3-12546]

Immunohistochemistry-Paraffin: Synaptotagmin 12 Antibody [NBP3-12546]

Immunohistochemistry-Paraffin: Synaptotagmin 12 Antibody [NBP3-12546] - Staining of human SYT12 in human kidney with rabbit polyclonal antibody at 1:100 dilution.
Immunoprecipitation: Synaptotagmin 12 Antibody [NBP3-12546]

Immunoprecipitation: Synaptotagmin 12 Antibody [NBP3-12546]

Immunoprecipitation: Synaptotagmin 12 Antibody [NBP3-12546] - Lane A:0.5 mg COS7 Whole Cell Lysate4 uL anti-SYT12 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-SYT12 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 46 kDaObserved band size :46 kDa

Applications for Synaptotagmin 12 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of uLysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Synaptotagmin 12

May be involved in ca(2+)-dependent exocytosis of secretory vesicles through ca(2+) and phospholipid binding to the c2 domain or may serve as ca(2+) sensors in the process of vesicular trafficking and exocytosis.

Alternate Names

SRG1, synaptotagmin XIIsynaptotagmin-XII, synaptotagmin-12, SYT11, sytXII

Gene Symbol

SYT12

Additional Synaptotagmin 12 Products

Product Documents for Synaptotagmin 12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Synaptotagmin 12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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