Syndecan-1/CD138 Antibody (B-A38)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64980

Clone B-A38 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB100-64980-0.02mg
NB100-64980-100ug
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Functional, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # B-A38

Concentration

0.1 mg/ml

View all Syndecan-1/CD138 Antibodies »

Product Specifications

Immunogen

U266 cell line

Localization

Membrane; Single-pass type I membrane protein.

Specificity

NB100-64980 recognizes a heparan sulfate rich membrane glycoprotein of 200kD known as Syndecan-1 (CD138). Syndecan functions as a receptor for collagen, fibronectin and thrombospondin. NB100-64980 stains all plasma cells and plasma cell lines. No staining of other leucocytes is seen in peripheral blood or bone marrow, although some ALL cell lines express the antigen weakly. Some staining of epithelial and endothelial cells may be seen in immunohistology.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Syndecan-1/CD138 Antibody (B-A38)

Flow Cytometry: Syndecan-1/CD138 Antibody (B-A38) [NB100-64980]

Flow Cytometry: Syndecan-1/CD138 Antibody (B-A38) [NB100-64980]

Flow Cytometry: Syndecan-1/CD138 Antibody (B-A38) [NB100-64980] - Staining of U266 cell line with MOUSE ANTI HUMAN CD138: FITC.
Syndecan-1/CD138 Antibody (B-A38)

Immunohistochemistry-Parafin: Mouse Monoclonal Syndecan-1/CD138 Antibody (B-A38) [NB100-64980] -

Immunohistochemistry-Parafin: Mouse Monoclonal Syndecan-1/CD138 Antibody (B-A38) [NB100-64980] - Syndecan-1/CD138 analysis on human lymph node tissue. Heat induced epitope retrieval-basic. Primary antibody x500 (0.2ug/mL). Image from verified customer review.

Applications for Syndecan-1/CD138 Antibody (B-A38)

Application
Recommended Usage

Flow Cytometry

Neat

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:100-1:2000
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. For IHC: This product requires heat pre-treatment. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Reviewed Applications

Read 1 review rated 5 using NB100-64980 in the following applications:

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syndecan-1/CD138

CD138 / Syndecan 1 is a transmembrane heparin sulphate proteoglycan which is made up of one core protein and five glycosaminoglycan. CD138 is expected to play a role in cell adhesion. It is expressed on the surface of pre B cells and plasma cells but is absent from mature B cells. It is a selective marker for B cell lymphoblastic leukemia and lymphoplasmocytoid leukemia. It is lost from the apoptotic myeloma cells; hence is a useful marker for viable myeloma cells. This antibody stains all plasma cells and plasma cell lines. No staining of other leucocytes is seen in peripheral blood or bone marrow, although some ALL cell lines express the antigen weakly. Some staining of epithelial and endothelial cells may be seen in immunohistology.

Alternate Names

CD138, SDC1, Syndecan1

Entrez Gene IDs

6382 (Human)

Gene Symbol

SDC1

UniProt

P18827 (Human)

Additional Syndecan-1/CD138 Products

Product Documents for Syndecan-1/CD138 Antibody (B-A38)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Syndecan-1/CD138 Antibody (B-A38)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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