Syndecan-1/CD138 Antibody (JM11-21)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67174

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67174
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM11-21

Concentration

1 mg/ml

View all Syndecan-1/CD138 Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human Syndecan-1/CD138 aa 13-274 / 310. (SwissProt: P18827 Human; SwissProt: P18828 Mouse; SwissProt: P26260 Rat)

Localization

Membrane, Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Syndecan-1/CD138 Antibody (JM11-21)

Western Blot: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174]

Western Blot: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174]

Western Blot: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Analysis of Syndecan 1 on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174]

Immunocytochemistry/ Immunofluorescence: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174]

Immunocytochemistry/Immunofluorescence: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Staining Syndecan 1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174]

Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174]

Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Analysis of paraffin-embedded mouse prostate tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.
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Applications for Syndecan-1/CD138 Antibody (JM11-21)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syndecan-1/CD138

CD138, also known as Syndecan-1, is a transmembrane heparin sulfate proteoglycan that binds type I collagen. The relative molecular mass of CD138 is found to vary between 20-95 kDa due to the presence of a variable number of GAG modifications on each CD138 molecule. This protein appears to mediate binding of cells to the extracellular matrix. Among mature tissues, CD138 is expressed on simple and stratified epithelia, endothelial cells, and fibroblasts. (2,3) While stratified keratinocytes express CD138, their malignant transformation suppresses CD138 expression. Wound repair processes alter patterns of CD138 expression. Among hematopoietic cells, CD138 is expressed by immunoglobulin-negative pre-B cells in the bone marrow, immature B cells and by terminally differentiated plasma cells in lymphoid organs. CD138 expression has not been observed on mature, circulating peripheral B lymphocytes, however. (4-7) CD138 is also strongly expressed by multiple myeloma-derived cell lines and elevated levels of CD138 are present in the serum of patients with multiple myeloma, a negative prognostic indicator for the disease. (8)

Alternate Names

CD138, SDC1, Syndecan1

Gene Symbol

SDC1

Additional Syndecan-1/CD138 Products

Product Documents for Syndecan-1/CD138 Antibody (JM11-21)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Syndecan-1/CD138 Antibody (JM11-21)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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