Syndecan-4 Antibody (PSH0-72)

Western Blot: Syndecan-4 Antibody (PSH0-72) [NBP3-32987]

Western Blot: Syndecan-4 Antibody (PSH0-72) [NBP3-32987] - Western blot analysis of Syndecan-4 on different lysates with Rabbit anti-Syndecan-4 antibody (NBP3-32987) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HeLa cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: A549 cell lysate
Lane 6: MCF7 cell lysate
Lane 7: MDA-MB-231 cell lysate

Lysates/proteins at 40 ug/Lane.

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 45 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32987) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.