Syntaxin 4 Antibody (JE35-92)

Western Blot: Syntaxin 4 Antibody (JE35-92) [NBP3-32989]

Western Blot: Syntaxin 4 Antibody (JE35-92) [NBP3-32989] - Western blot analysis of Syntaxin 4 on different lysates with Rabbit anti-Syntaxin 4 antibody (NBP3-32989) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate (20 ug/Lane)
Lane 2: A549 cell lysate (20 ug/Lane)
Lane 3: Jurkat cell lysate (20 ug/Lane)
Lane 4: HeLa cell lysate (20 ug/Lane)
Lane 5: K-562 cell lysate (20 ug/Lane)
Lane 6: U-87 MG cell lysate (20 ug/Lane)
Lane 7: Human lung tissue lysate (40 ug/Lane)
Lane 8: Mouse skin tissue lysate (40 ug/Lane)
Lane 9: rat brain tissue lysate (40 ug/Lane)

Predicted band size: 34 kDa
Observed band size: 34/30 kDa

Exposure time: 15 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32989) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Syntaxin 4 Antibody (JE35-92) [NBP3-32989]

Immunohistochemistry: Syntaxin 4 Antibody (JE35-92) [NBP3-32989] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Syntaxin 4 antibody (NBP3-32989) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32989) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.