Syntaxin 4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87374

Novus Biologicals, part of Bio-Techne
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NBP1-87374
NBP1-87374-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Syntaxin 4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MRDRTHELRQGDDSSDEEDKERVALVVHPGTARLGSPDEEFFHKVRTIRQTIVKLGNKVQELEKQQVTILATPLPEESMKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVNTRMRKTQHGVLSQQFVELINKCNSMQSE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Syntaxin 4 Antibody

Western Blot: Syntaxin 4 Antibody [NBP1-87374]

Western Blot: Syntaxin 4 Antibody [NBP1-87374]

Western Blot: Syntaxin 4 Antibody [NBP1-87374] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-STX4 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: Syntaxin 4 Antibody [NBP1-87374]

Immunocytochemistry/ Immunofluorescence: Syntaxin 4 Antibody [NBP1-87374]

Immunocytochemistry/Immunofluorescence: Syntaxin 4 Antibody [NBP1-87374] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Syntaxin 4 Antibody [NBP1-87374]

Immunohistochemistry-Paraffin: Syntaxin 4 Antibody [NBP1-87374]

Immunohistochemistry-Paraffin: Syntaxin 4 Antibody [NBP1-87374] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.
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Applications for Syntaxin 4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syntaxin 4

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes . May also play a role in docking of synaptic vesicles at presynaptic active zones

Alternate Names

NY-REN-31, p35-2, STX4

Entrez Gene IDs

6810 (Human)

Gene Symbol

STX4

UniProt

Q12846

Additional Syntaxin 4 Products

Product Documents for Syntaxin 4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Syntaxin 4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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