Syntaxin 7 Antibody (CL0257)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52893
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (97%), Rat (94%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0257
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Syntaxin 7 Antibody (CL0257)
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GVGGDPAQLAQRISSNIQKITQCSVEIQRTLNQLGTPQDSPELRQQLQQKQQYTNQLAKETDKYIKEFGSLPTTPSEQRQRKIQKDRLVAEFTTSLTNFQKVQRQAAEREKEFVARVRASSRVSGSFPEDSSKERNLVSWESQTQPQV
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Syntaxin 7 Antibody (CL0257)
![Western Blot: Syntaxin 7 Antibody (CL0257) [NBP2-52893]](https://resources.bio-techne.com/images/products/Syntaxin-7-Antibody-CL0257-Western-Blot-NBP2-52893-img0004.jpg "Western Blot: Syntaxin 7 Antibody (CL0257) [NBP2-52893]")
Western Blot: Syntaxin 7 Antibody (CL0257) [NBP2-52893]
Western Blot: Syntaxin 7 Antibody (CL0257) [NBP2-52893] - Lane 1: Marker [kDa], Lane 2: Human tonsil tissue lysate![Immunocytochemistry/ Immunofluorescence: Syntaxin 7 Antibody (CL0257) [NBP2-52893]](https://resources.bio-techne.com/images/products/Syntaxin-7-Antibody-CL0257-Immunocytochemistry-Immunofluorescence-NBP2-52893-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Syntaxin 7 Antibody (CL0257) [NBP2-52893]")
Immunocytochemistry/ Immunofluorescence: Syntaxin 7 Antibody (CL0257) [NBP2-52893]
Immunocytochemistry/Immunofluorescence: Syntaxin 7 Antibody (CL0257) [NBP2-52893] - Staining of U-251 cells using the Anti-STX7 monoclonal antibody, showing specific staining in vesicles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Applications for Syntaxin 7 Antibody (CL0257)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Syntaxin 7
Alternate Names
STX7
Gene Symbol
STX7
Additional Syntaxin 7 Products
Product Documents for Syntaxin 7 Antibody (CL0257)
Product Specific Notices for Syntaxin 7 Antibody (CL0257)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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