Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B6

Format

BSA Free

Concentration

1.0 mg/ml

View all Synuclein alpha/beta Antibodies »

Product Summary for Synuclein alpha/beta Antibody (3B6) - BSA Free

Immunogen

This Synuclein alpha/beta Antibody (3B6) was developed against recombinant human Synuclein alpha/beta (119-140aa) purified from E. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Synuclein alpha/beta Antibody (3B6) - BSA Free

Immunocytochemistry/ Immunofluorescence: Synuclein alpha/beta Antibody (3B6) - BSA Free [NBP1-22957]

Immunocytochemistry/ Immunofluorescence: Synuclein alpha/beta Antibody (3B6) - BSA Free [NBP1-22957]

Immunocytochemistry/Immunofluorescence: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of LNCaP cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human Synuclein alpha/beta Antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western Blot: Synuclein alpha/beta Antibody (3B6)BSA Free [NBP1-22957]

Western Blot: Synuclein alpha/beta Antibody (3B6)BSA Free [NBP1-22957]

Western Blot: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - The recombinant human synuclein family (alpha-,beta- and gamma-) (each 20ng), Mouse brain and Rat brain (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human alpha,beta-synuclein (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Flow Cytometry: Synuclein alpha/beta Antibody (3B6) - BSA Free [NBP1-22957]

Flow Cytometry: Synuclein alpha/beta Antibody (3B6) - BSA Free [NBP1-22957]

Flow Cytometry: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of alpha, beta-Synuclein in C6 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Applications for Synuclein alpha/beta Antibody (3B6) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

2-5ug

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Synuclein alpha/beta

Alternate Names

beta-synuclein, I(2)-synuclein, I+/--synuclein, NACP, non A-beta component of AD amyloid, PARK1, PD1, synuclein alpha-140, synuclein, alpha (non A4 component of amyloid precursor), truncated alpha synuclein

Entrez Gene IDs

6622 (Human)

Gene Symbol

SNCA

UniProt

NP_000336 (Human)

Additional Synuclein alpha/beta Products

Product Documents for Synuclein alpha/beta Antibody (3B6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Synuclein alpha/beta Antibody (3B6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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