Synuclein alpha/beta Antibody (9E9X5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33536
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 9E9X5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Synuclein alpha/beta (P37840).
Sequence:
MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQL
Sequence:
MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Synuclein alpha/beta Antibody (9E9X5)
Western Blot: Synuclein alpha/beta Antibody (9E9X5) [NBP3-33536] -
Western Blot: Synuclein alpha/beta Antibody (9E9X5) [NBP3-33536] - Western blot analysis of lysates from U-87MG cells, using Synuclein alpha/beta Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: Synuclein alpha/beta Antibody (9E9X5) [NBP3-33536] -
Immunocytochemistry/ Immunofluorescence: Synuclein alpha/beta Antibody (9E9X5) [NBP3-33536] - Immunofluorescence analysis of paraffin-embedded rat brain using Synuclein alpha/beta Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western Blot: Synuclein alpha/beta Antibody (9E9X5) [NBP3-33536] -
Western Blot: Synuclein alpha/beta Antibody (9E9X5) [NBP3-33536] - Western blot analysis of various lysates using Synuclein alpha/beta Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1min.
Applications for Synuclein alpha/beta Antibody (9E9X5)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Synuclein alpha/beta
Alternate Names
beta-synuclein, I(2)-synuclein, I+/--synuclein, NACP, non A-beta component of AD amyloid, PARK1, PD1, synuclein alpha-140, synuclein, alpha (non A4 component of amyloid precursor), truncated alpha synuclein
Gene Symbol
SNCA
Additional Synuclein alpha/beta Products
Product Documents for Synuclein alpha/beta Antibody (9E9X5)
Product Specific Notices for Synuclein alpha/beta Antibody (9E9X5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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