SYPL1 Antibody (EP10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59340
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # EP10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Immunoprecipitate of human brain
Localization
Cytoplasmic Vesicle , Secretory Vesicle , Synaptic Vesicle Membrane , Cell Junction , Synapse
Specificity
Detects 38kDa.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for SYPL1 Antibody (EP10)
Western Blot: SYPL1 Antibody (EP10) [NBP2-59340]
Western Blot: SYPL1 Antibody (EP10) [NBP2-59340] - Analysis of Human Brain showing detection of ~38 kDa Synaptophysin protein using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~38 kDa. Other Band(s): 25 kDa, 20 kDa.Immunohistochemistry: SYPL1 Antibody (EP10) [NBP2-59340]
Immunohistochemistry: SYPL1 Antibody (EP10) [NBP2-59340] - Tissue: Cerebellum. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Neurons and fibers. Magnification: 20x.Immunohistochemistry: SYPL1 Antibody (EP10) [NBP2-59340]
Immunohistochemistry: SYPL1 Antibody (EP10) [NBP2-59340] - Tissue: Brain Slice. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Neurons and fibers but not on the white matter. Magnification: 20x.Applications for SYPL1 Antibody (EP10)
Application
Recommended Usage
Immunohistochemistry
1:10 - 1:500
Western Blot
1:100 - 1:2000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: SYPL1
Alternate Names
H-SP1, pantophysin, synaptophysin-like 1, synaptophysin-like protein, synaptophysin-like protein 1, SYPL
Gene Symbol
SYPL1
Additional SYPL1 Products
Product Documents for SYPL1 Antibody (EP10)
Product Specific Notices for SYPL1 Antibody (EP10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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