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T-bet/TBX21 Antibody (BLR110H)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14699

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14699

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR110H

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

between 485 and 535 (C-term)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for T-bet/TBX21 Antibody (BLR110H)

Immunoprecipitation: T-bet/TBX21 Antibody (BLR110H) [NBP3-14699]

Immunoprecipitation: T-bet/TBX21 Antibody (BLR110H) [NBP3-14699]

Immunoprecipitation: T-bet/TBX21 Antibody [NBP3-14699] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from MJ/G11 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiT-bet/TBX21 recombinant monoclonal antibody used for IP at 6 ul per reaction. Tbet/TBX21 was also immunoprecipitated by rabbit anti-Tbet/TBX21 antibody. For blottingimmunoprecipitated T-bet/TBX21, was used at1:1000. Chemiluminescence with an exposure time of 3seconds.

Applications for T-bet/TBX21 Antibody (BLR110H)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with , BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: T-bet/TBX21

Tbx21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq]

Long Name

T-box transcription factor TBX21

Alternate Names

T-PET, Tbet, TBLYM, TBX21

Gene Symbol

TBX21

Additional T-bet/TBX21 Products

Product Documents for T-bet/TBX21 Antibody (BLR110H)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for T-bet/TBX21 Antibody (BLR110H)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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