Key Product Details

Species Reactivity

Validated:

Epitope Tag

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all T7 Epitope Tag Antibodies »

Product Specifications

Immunogen

Goats were immunized with T7 cleavage site (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 27226551).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for T7 Epitope Tag Antibody - BSA Free

Western Blot: T7 Epitope Tag AntibodyBSA Free [NB600-371]

Western Blot: T7 Epitope Tag AntibodyBSA Free [NB600-371]

Western Blot: T7 Epitope Tag Antibody [NB600-371] - Western Blot: T7 Epitope Tag Antibody [HRP] [NB600-377] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000). Image using the HRP form of this antibody.
Western Blot: T7 Epitope Tag AntibodyBSA Free [NB600-371]

Western Blot: T7 Epitope Tag AntibodyBSA Free [NB600-371]

Western Blot: T7 Epitope Tag Antibody [NB600-371] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).

Applications for T7 Epitope Tag Antibody - BSA Free

Application
Recommended Usage

ELISA

Primary-1:1000-1:30000; Coating: 1:100-1:500

Immunocytochemistry/ Immunofluorescence

1:100-1:400

Western Blot

1:1000-1:10000
Application Notes
Suggested working dilutions: * ELISA Coating 1:100-1:500Primary 1:1000-1:30,000 Western Blots Colorimetric detection 1:1000-1:10,000ECL 1:1000-1:30,000 cyto 1:100-1:400+ (see reference) *The investigator should determine the optimal working dilution for a specific application. ELISA, Western blot and immunocytochemistry.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: T7 Epitope Tag

The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.

Alternate Names

MASMTGGQQMG epitope tag, MASMTGGQQMG tag, T7 epitope tag

Additional T7 Epitope Tag Products

Product Documents for T7 Epitope Tag Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for T7 Epitope Tag Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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