TACE/ADAM17 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77044

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human, Rat

Predicted:

Mouse (94%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Block/Neutralize

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all TACE/ADAM17 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human TACE. The immunogen is located within the last 50 amino acids of TACE. Amino Acid Squence: ASFKLQRQNRVDSKETE

Specificity

80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE/ADAM17. Human TACE/ADAM17 has 2 isoforms, including isoform A (824aa, 93kD), isoform B (694aa, 78.5kD). This antibody detects only isoform A. Mouse TACE/ADAM17 has 2 isoforms, long isoform (827aa, 93.1kD) and short isoform (655aa, 73.9kD). Rat TACE/ADAM17 has only one isoform identified so far (827aa, 93kD).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TACE/ADAM17 Antibody - BSA Free

Western Blot: TACE/ADAM17 AntibodyBSA Free [NBP1-77044]

Western Blot: TACE/ADAM17 AntibodyBSA Free [NBP1-77044]

Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysates or TACE KO celllysates. Antibodies: TACE/ADAM17 NBP1-77044 (0.25 ug/mL) and beta-actin NBP1-76692 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunocytochemistry/ Immunofluorescence: TACE/ADAM17 Antibody - BSA Free [NBP1-77044]

Immunocytochemistry/ Immunofluorescence: TACE/ADAM17 Antibody - BSA Free [NBP1-77044]

Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody [NBP1-77044] - HeLa cells with TACE antibody at 10 ug/mL.
Western Blot: TACE/ADAM17 AntibodyBSA Free [NBP1-77044]

Western Blot: TACE/ADAM17 AntibodyBSA Free [NBP1-77044]

Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - HeLa whole cell lysate. Image from verified customer review.
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Applications for TACE/ADAM17 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Western Blot

0.25-2 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using NBP1-77044 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TACE/ADAM17

Tumor-necrosis factor-alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-alpha is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-alpha. TNF-alpha converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.

Long Name

TNF-alpha Converting Enzyme

Alternate Names

ADAM17, CD156b

Gene Symbol

ADAM17

UniProt

NP_003174 (Human)

Additional TACE/ADAM17 Products

Product Documents for TACE/ADAM17 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TACE/ADAM17 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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