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TADA3L Antibody (3H3)

Novus Biologicals, part of Bio-Techne | Catalog # H00010474-M02

Novus Biologicals, part of Bio-Techne
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H00010474-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3H3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

TADA3L (AAH13433, 1 a.a. ~ 432 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSELKDCPLQFHDFKSVDHLKVCPRYTAVLARSEDDGIGIEELDTLQLELETLLSSASRRLRVLEAETQILTDWQDKKGDRRFLKLGRDHELGAPPKHGKPKKQKLEGKAGHGPGPGPGRPKSKNLQPKIQEYEFTDDPIDVPRIPKNDAPNRFWASVEPYCADITSEEVRTLEELLKPPEDEAEHYKIPPLGKHYSQRWAQEDLLEEQKDGARAAAVADKKKGLMGPLTELDTKDVDALLKKSEAQHEQPEDGCPFGALTQRLLQALVEENIISPMEDSPIPDMSGKESGADGASTSPRNQNKPFSVPHTKSLESRIKEELIAQGLLESEDRPAEDSEDEVLAELRKRQAELKALSAHNRTKKHDLLRLAKEEVSRQELRQRVRMADNEVMDAFRKIMAARQKKRTPTKKEKDQAWKTLKERESILKLLDG

Specificity

TADA3L - transcriptional adaptor 3 (NGG1 homolog, yeast)-like (3H3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for TADA3L Antibody (3H3)

Western Blot: TADA3L Antibody (3H3) [H00010474-M02]

Western Blot: TADA3L Antibody (3H3) [H00010474-M02]

Western Blot: TADA3L Antibody (3H3) [H00010474-M02] - Analysis of TADA3L expression in transfected 293T cell line by TADA3L monoclonal antibody (M02), clone 3H3.Lane 1: TADA3L transfected lysate(41.4 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: TADA3L Antibody (3H3) [H00010474-M02] - Detection limit for recombinant GST tagged TADA3L is 1 ng/ml as a capture antibody.

Applications for TADA3L Antibody (3H3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TADA3L

Many DNA-binding transcriptional activator proteins enhance the initiation rate of RNA polymerase II-mediated gene transcription by interacting functionally with the general transcription machinery bound at the basal promoter. Adaptor proteins are usually required for this activation, possibly to acetylate and destabilize nucleosomes, thereby relieving chromatin constraints at the promoter. The protein encoded by this gene is a transcriptional activator adaptor and has been found to be part of the PCAF histone acetylase complex. In addition, it associates with the tumor suppressor protein p53 and is required for full activity of p53 and p53-mediated apoptosis. At least four alternatively spliced variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq]

Alternate Names

ADA3, ADA3 homolog, alteration/deficiency in activation 3, epididymis secretory sperm binding protein, FLJ20221, FLJ21329, hADA3, NGG1, STAF54, TADA3L, transcriptional adapter 3, Transcriptional adapter 3-like, transcriptional adaptor 3

Entrez Gene IDs

10474 (Human)

Gene Symbol

TADA3

Additional TADA3L Products

Product Documents for TADA3L Antibody (3H3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TADA3L Antibody (3H3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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