Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all TAF15 Antibodies »

Product Specifications

Immunogen

A synthetic peptide which maps to a region between residues 550 and the C-terminus (residue 592) of human TAFII68 using the numbering given in Swiss-Prot entry Q92804 (GeneID 8148).

Specificity

NB100-568 is specific for human TAFII-68 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TAF15 Antibody - BSA Free

Western Blot: TAF15 Antibody [NB100-568]

Western Blot: TAF15 Antibody [NB100-568]

Western Blot: TAF15 Antibody [NB100-568] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAFII68 antibody A used for WB at 0.033 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: TAF15 Antibody [NB100-568]

Immunoprecipitation: TAF15 Antibody [NB100-568]

Immunoprecipitation: TAF15 Antibody [NB100-568] - Detection of human TAFII68 on HeLa whole cell lysate using NB100-568. TAFII68 was IPed with rabbit anti-TAFII68 antibodies NB100-566, NB100-567, NB100-568, and another using each at 0.3 ug/mg lysate.

Applications for TAF15 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1 -4 ug/mg lysate

Western Blot

1:10000 - 1:30000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TAF15

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation.

Alternate Names

68kDa, hTAFII68, Npl3, RBP56TAFII68, RNA-binding protein 56, TAF(II)68, TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, TAF2NRBP56/CSMF fusion, TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD(RNA-binding protein 56), TATA box-binding protein-associated factor 2N (RNA-binding protein 56), TATA-binding protein-associated factor 2N, TBP-associated factor 15,68 kDa TATA-binding protein-associated factor

Entrez Gene IDs

8148 (Human)

Gene Symbol

TAF15

UniProt

Q92804 (Human)

Additional TAF15 Products

Product Documents for TAF15 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TAF15 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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