TAK1 Antibody (2E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00006885-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TAK1 Antibodies »

Product Specifications

Immunogen

MAP3K7 (AAH17715, 1 a.a. ~ 579 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSTASAASSSSSSSAGEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYFPGADEPLQYPCQYSDEGQSNSATSTGSFMDIASTNTSNKSDTNMEQVPATNDTIKRLESKLLKNQAKQQSESGRLSLGASRGSSVESLPPTSEGKRMSADMSEIEARIAATTGNGQPRRRSIQDLTVTGTEPGQVSSRSSSPSVRMITTSGPTSEKPTRSHPWTPDDSTDTNGSDNSIPMAYLTLDHQLQPLAPCPNSKESMAVFEQHCKMAQEYMKVQTEIALLLQRKQELVAELDQDEKDQQNTSRLVQEHKKLLDENKSLSTYYQQCKKQLEVIRSQQQKRQGTS

Localization

Cytoplasmic

Specificity

MAP3K7 - mitogen-activated protein kinase kinase kinase 7

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TAK1 Antibody (2E10)

Western Blot: TAK1 Antibody (2E10) [H00006885-M02]

Western Blot: TAK1 Antibody (2E10) [H00006885-M02]

Western Blot: TAK1 Antibody (2E10) [H00006885-M02] - Analysis of MAP3K7 expression in transfected 293T cell line by MAP3K7 monoclonal antibody (M02), clone 2E10.Lane 1: MAP3K7 transfected lysate(63.8 KDa).Lane 2: Non-transfected lysate.
Western Blot: TAK1 Antibody (2E10) [H00006885-M02]

Western Blot: TAK1 Antibody (2E10) [H00006885-M02]

Western Blot: TAK1 Antibody (2E10) [H00006885-M02] - Analysis of MAP3K7 over-expressed 293 cell line, cotransfected with MAP3K7 Validated Chimera RNAi ( Cat # H00006885-R02V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAP3K7 monoclonal antibody (M02) clone 2E10 (Cat # H00006885-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
ELISA: TAK1 Antibody (2E10) [H00006885-M02]

ELISA: TAK1 Antibody (2E10) [H00006885-M02]

ELISA: TAK1 Antibody (2E10.) [H00006885-M02] - TAK1 Antibody (2E10) [H00006885-M02] - Detection limit for recombinant GST tagged MAP3K7 is approximately 1ng/ml as a capture antibody.

Applications for TAK1 Antibody (2E10)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA. It has been used for RNAi Validation and WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TAK1

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Long Name

TGF-beta activated kinase 1

Alternate Names

MAP3K7, TGF1a

Entrez Gene IDs

6885 (Human)

Gene Symbol

MAP3K7

OMIM

602614 (Human)

UniProt

AAH17715 (Human)

Additional TAK1 Products

Product Documents for TAK1 Antibody (2E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TAK1 Antibody (2E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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