TATA binding protein TBP Antibody - Isoform 1

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12932

Novus Biologicals, part of Bio-Techne
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NBP2-12932
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all TATA binding protein TBP Antibodies »

Product Specifications

Immunogen

Peptide with sequence DQNNSLPPYAQ-C corresponding to N-Terminus according to NP_003185.1.

Epitope

DQNNSLPPYAQC

Specificity

This antibody is expected to recognize reported isoform 1(NP_003185.1) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TATA binding protein TBP Antibody - Isoform 1

Western Blot: TATA binding protein TBP AntibodyIsoform 1 [NBP2-12932]

Western Blot: TATA binding protein TBP AntibodyIsoform 1 [NBP2-12932]

Western Blot: TATA binding protein TBP isoform 1 Antibody [NBP2-12932] - TATA binding protein TBP isoform 1 Antibody 0.3ug/ml staining of HeLa nuclear (A) and cytosolic (B) lysates (35ug protein in commercial extraction buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for TATA binding protein TBP Antibody - Isoform 1

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in nuclear, but not in cytosolic HeLa lysates (calculated MW of 37.7 kDa band according to NP_003185.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TATA binding protein TBP

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein TBP and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.TPB is also known to interact with HIV1 Tat protein.

Alternate Names

GTF2D, GTF2D1, HDL4, MGC126054, MGC126055, SCA17, TATA box binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box binding protein N-terminal domain, TATA-box factor, TATA-box-binding protein, TF2D, TFIIDMGC117320, Transcription initiation factor TFIID TBP subunit

Entrez Gene IDs

6908 (Human); 21374 (Mouse); 117526 (Rat)

Gene Symbol

TBP

Additional TATA binding protein TBP Products

Product Documents for TATA binding protein TBP Antibody - Isoform 1

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TATA binding protein TBP Antibody - Isoform 1

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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