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TATA Element Modulatory Factor 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90117

Novus Biologicals, part of Bio-Techne
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NBP1-90117-25ul
NBP1-90117

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TSTTSDIEVLDHESVISESSASSRQETTDSKSSLHLMQTSFQLLSASACPEYNRLDDFQKLTESCCSSDAFERIDSFSVQSLDSRSVSEINSDDELSGKGYALVPIIVNSSTPKSKTVESAEGKSEEVNETLVIPTEEAEMEESG

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22553199)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TATA Element Modulatory Factor 1 Antibody

Western Blot: TATA Element Modulatory Factor 1 Antibody [NBP1-90117]

Western Blot: TATA Element Modulatory Factor 1 Antibody [NBP1-90117]

Western Blot: TATA Element Modulatory Factor 1 Antibody [NBP1-90117] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TMF1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: TATA Element Modulatory Factor 1 Antibody [NBP1-90117]

Immunocytochemistry/ Immunofluorescence: TATA Element Modulatory Factor 1 Antibody [NBP1-90117]

Immunocytochemistry/Immunofluorescence: TATA Element Modulatory Factor 1 Antibody [NBP1-90117] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TATA Element Modulatory Factor 1 Antibody [NBP1-90117]

Immunohistochemistry-Paraffin: TATA Element Modulatory Factor 1 Antibody [NBP1-90117]

Immunohistochemistry-Paraffin: TATA Element Modulatory Factor 1 Antibody [NBP1-90117] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.

Applications for TATA Element Modulatory Factor 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TATA Element Modulatory Factor 1

Potential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. This protein binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP)

Alternate Names

TATA element modulatory factor 1

Gene Symbol

TMF1

Additional TATA Element Modulatory Factor 1 Products

Product Documents for TATA Element Modulatory Factor 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TATA Element Modulatory Factor 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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