Tau Antibody (2E9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25162
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Equine
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Proximity Ligation Assay, Western Blot
Cited:
Immunohistochemistry, Proximity Ligation Assay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2E9
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant full length 441 amino acid human tau. Epitope located in the peptide KDRVQSKIGSLDNITHVPGG, amino acids 347-366.
Localization
Cytoplasm > cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm > cytoskeleton. Cell projection > axon. Note: Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
Specificity
This antibody is expected to bind to all Tau isoforms
Marker
pan-Neuronal Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
48-67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Tau Antibody (2E9)
Western Blot: Tau Antibody (2E9) [NBP2-25162]
Western Blot: Tau Antibody (2E9) [NBP2-25162] - Analysis of different tissue lysates using mouse mAb to MAP-tau, NBP2-25162, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as up to 9 different isoforms of different molecular weight, and so appears as multiple closely spaced bands in the range from 48 kDa to 67 kDa in the CNS and including larger big tau forms in the PNS, visible in lane 5.Immunocytochemistry/ Immunofluorescence: Tau Antibody (2E9) [NBP2-25162]
Immunocytochemistry/Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - Analysis of cortical neuron-glial culture from E20 rat stained with mouse mAb to MAP-tau, NBP2-25162, dilution 1:1,000 in green, and costained with chicken pAb to MAP2, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA. NBP2-25162 antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both proteins.Immunocytochemistry/ Immunofluorescence: Tau Antibody (2E9) [NBP2-25162]
Immunocytochemistry/Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - E18 primary rat hippocampal neurons (from Neuromics) were fixed and immunostained with NBP2-25162 (green), chicken antibody to alpha Internexin (NBP1-05208, red) and for DNA (blue). NBP2-25162 stains the neuronal perikarya and processes strongly, and does not stain non neuronal cells in these cultures. The alpha Internexin antibody stains intermediate or 10nm filament bundles in the cytoplasm of these cells.Applications for Tau Antibody (2E9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000
Immunohistochemistry
1:1000
Western Blot
1:10000
Application Notes
Use in PLA reported in scientific literature (PMID:35264729) Optimal dilution of this antibody should be experimentally determined..
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50% PBS, 50% glycerol
Preservative
0.035% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Tau
Long Name
Microtubule-Associated Protein Tau
Alternate Names
MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND
Entrez Gene IDs
4137 (Human)
Gene Symbol
MAPT
OMIM
153619 (Human)
UniProt
Additional Tau Products
Product Documents for Tau Antibody (2E9)
Product Specific Notices for Tau Antibody (2E9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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