Tau Antibody (2E9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25162

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Equine

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Proximity Ligation Assay, Western Blot

Cited:

Immunohistochemistry, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2E9

Concentration

1 mg/ml

View all Tau Antibodies »

Product Specifications

Immunogen

Recombinant full length 441 amino acid human tau. Epitope located in the peptide KDRVQSKIGSLDNITHVPGG, amino acids 347-366.

Localization

Cytoplasm > cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm > cytoskeleton. Cell projection > axon. Note: Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.

Specificity

This antibody is expected to bind to all Tau isoforms

Marker

pan-Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48-67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tau Antibody (2E9)

Western Blot: Tau Antibody (2E9) [NBP2-25162]

Western Blot: Tau Antibody (2E9) [NBP2-25162]

Western Blot: Tau Antibody (2E9) [NBP2-25162] - Analysis of different tissue lysates using mouse mAb to MAP-tau, NBP2-25162, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as up to 9 different isoforms of different molecular weight, and so appears as multiple closely spaced bands in the range from 48 kDa to 67 kDa in the CNS and including larger big tau forms in the PNS, visible in lane 5.
Immunocytochemistry/ Immunofluorescence: Tau Antibody (2E9) [NBP2-25162]

Immunocytochemistry/ Immunofluorescence: Tau Antibody (2E9) [NBP2-25162]

Immunocytochemistry/Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - Analysis of cortical neuron-glial culture from E20 rat stained with mouse mAb to MAP-tau, NBP2-25162, dilution 1:1,000 in green, and costained with chicken pAb to MAP2, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA. NBP2-25162 antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both proteins.
Immunocytochemistry/ Immunofluorescence: Tau Antibody (2E9) [NBP2-25162]

Immunocytochemistry/ Immunofluorescence: Tau Antibody (2E9) [NBP2-25162]

Immunocytochemistry/Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - E18 primary rat hippocampal neurons (from Neuromics) were fixed and immunostained with NBP2-25162 (green), chicken antibody to alpha Internexin (NBP1-05208, red) and for DNA (blue). NBP2-25162 stains the neuronal perikarya and processes strongly, and does not stain non neuronal cells in these cultures. The alpha Internexin antibody stains intermediate or 10nm filament bundles in the cytoplasm of these cells.

Applications for Tau Antibody (2E9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:10000
Application Notes
Use in PLA reported in scientific literature (PMID:35264729) Optimal dilution of this antibody should be experimentally determined..
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tau

Tau is a relatively low molecular weight member of the microtubule associated protein or MAP family. Most of these proteins were discovered since microtubules can be polymerized in cell homogenates and pelleted out by centrifugation, typically taking MAP proteins with them. This early work showed that the Tau protein facilitated the polymerization of microtubules, and was therefore named after the Greek letter tau, since it promoted tubule formation. Tau is heavily concentrated in axons of neurons, but may also be found in dendrites and in some non-neuronal cells. Much interest has focused on Tau as it is a major component of the neurofibrillary tangles of Alzheimer's disease. Tau in neurofibrillary tangles is typically heavily and aberrantly phosphorylated, and it is believed that phosphorylation may be involved in tangle formation. In addition, numerous different point mutations in the Tau gene are causative of Frontotemporal dementia with Parkinsonism linked to chromosome 17 (FTDP-17). There is one mammalian Tau gene which produces at least 9 different proteins by alternate transcription. In the central nervous system 6 isoforms predominant which either include or do not include three short exon coded inserts. These proteins range in size from 352-441 amino acids and run on SDS-PAGE gels as multiple bands ranging from 48-67 kDa. In peripheral nervous system a form called "big tau" predominates, another alternate transcript which includes a 254 amino acid insert. This form of Tau is found in small amounts in the brain also, in cranial nerve motor nuclei and sensory processes of most sensory ganglia, and runs on SDS-PAGE with an apparent molecular weight of 100 kDa. Each Tau protein contains 3 or 4 copies of an 18 amino acid peptide which are responsible for binding to the microtubules and are similar to those found in MAP2 and other members of the MAP family. Tau is a highly charged acidic protein with few hydrophobic residues which belongs to the family of "intrinsically unstructured proteins". As with GAP43, MARCKS and several other similar proteins, Tau isoforms run on SDS-PAGE much more slowly than expected from their actual molecular weight. This antibody was made against a recombinant construct expressed in and purified from E. coli and which corresponded to the shortest version of the various Tau proteins, so this antibody is expected to bind to all Tau isoforms.

Long Name

Microtubule-Associated Protein Tau

Alternate Names

MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND

Entrez Gene IDs

4137 (Human)

Gene Symbol

MAPT

OMIM

153619 (Human)

UniProt

P10636

Additional Tau Products

Product Documents for Tau Antibody (2E9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Tau Antibody (2E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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