Tau Antibody (Tau-5) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81091

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Sheep

Applications

Electron Microscopy, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # Tau-5

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Tau Antibodies »

Product Specifications

Immunogen

Tau protein.

Specificity

This antibody binds to sequence 210-241 of tau protein.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Tau Antibody (Tau-5) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Tau Antibody (Tau-5) - Chimeric - Azide and BSA Free [NBP2-81091]

Immunocytochemistry/ Immunofluorescence: Tau Antibody (Tau-5) - Chimeric - Azide and BSA Free [NBP2-81091]

Immunocytochemistry/Immunofluorescence: Tau Antibody (Tau-5) [NBP2-81091] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of Tau-5 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81091, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.

Applications for Tau Antibody (Tau-5) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Electron Microscopy

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to tau in a phosphorylation independent manner. Tau proteins are abundant in the central nervous system and stabilize micrtubules. Immuno-staining can be affected by different fixation methods (see LoPresti et al. 1995).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Tau

Tau is a microtubule binding protein that promotes microtubule assembly and stability. Tau is found to be the major component of the paired helical filaments (PHFs) found in the brains of patients with Alzheimer disease (AD) (1,2). Tau is hyperphosphorylated in PHFs, and specific phosphorylation sites have been implicated in the loss of Tau's association with the membrane cortex during AD disease state, including Ser 199/202, Thr 231, and Ser 393/404 (3). Glycogen synthase kinase-3, or GSK-3, phosphorylates Tau on Ser 396 (4).

Long Name

Microtubule-Associated Protein Tau

Alternate Names

MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND

Gene Symbol

MAPT

Additional Tau Products

Product Documents for Tau Antibody (Tau-5) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Tau Antibody (Tau-5) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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