TBC1D10A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61998

Novus Biologicals, part of Bio-Techne
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NBP2-61998
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all TBC1D10A Antibodies »

Product Specifications

Immunogen

Peptide with sequence AKSNGENGPRAPA-C, from the N Terminus of the protein sequence according to NP_114143; NP_001191169.1.

Epitope

AKSNGENGPRAPA-C

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TBC1D10A Antibody

Immunocytochemistry/ Immunofluorescence: TBC1D10A Antibody [NBP2-61998]

Immunocytochemistry/ Immunofluorescence: TBC1D10A Antibody [NBP2-61998]

Immunocytochemistry/Immunofluorescence: TBC1D10A Antibody [NBP2-61998] - Paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic/Plasma Membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).
Immunohistochemistry-Paraffin: TBC1D10A Antibody [NBP2-61998]

Immunohistochemistry-Paraffin: TBC1D10A Antibody [NBP2-61998]

Immunohistochemistry-Paraffin: TBC1D10A Antibody [NBP2-61998] - (4.75ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Flow Cytometry: TBC1D10A Antibody [NBP2-61998]

Flow Cytometry: TBC1D10A Antibody [NBP2-61998]

Flow Cytometry: TBC1D10A Antibody [NBP2-61998] - Paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for TBC1D10A Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

4.75 ug/ml

Immunohistochemistry-Paraffin

4.75 ug/ml

Peptide ELISA

Detection Limit 1:2000
Application Notes
Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ plasma membrane and nucleus of MCF7 cells.
Flow Cytometry: Flow cytometric analysis of HEK293 cells.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TBC1D10A

Alternate Names

EBP50-PDX interactor of 64 kDa, EPI64, rab27A-GAP-alpha, TBC1 domain family member 10A, TBC1 domain family, member 10A

Gene Symbol

TBC1D10A

Additional TBC1D10A Products

Product Documents for TBC1D10A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TBC1D10A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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