Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TBC1D9 Antibodies »

Product Summary for TBC1D9 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 250 and 300 of human TBC1 domain family, member 9 using the numbering given in entry NP_055945.1 (GeneID 23158).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TBC1D9 Antibody

Western Blot: TBC1D9 Antibody [NB100-55320]

Western Blot: TBC1D9 Antibody [NB100-55320]

Western Blot: TBC1D9 Antibody [NB100-55320] - Detection of Human TBC1D9/MDR1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TBC1D9/MDR1 antibody NB100-55320 used for IP at 3 mcg/mg lysate. TBC1D9/MDR1 was also immunoprecipitated by rabbit anti-TBC1D9/MDR1 antibody NB100-55321, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: TBC1D9 Antibody [NB100-55320]

Immunoprecipitation: TBC1D9 Antibody [NB100-55320]

Immunoprecipitation: TBC1D9 Antibody [NB100-55320] - Detection of human TBC1D9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBC1D9 antibody NB100-55320 used for IP at 6 ug per reaction. TBC1D9 was also immunoprecipitated by rabbit anti-TBC1D9 antibody NB100-55321. For blotting immunoprecipitated TBC1D9, NB100-55321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for TBC1D9 Antibody

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TBC1D9

TBC1D9, also known as MDR1 (multidrug resistance 1), is a P-glycoprotein implicated in the mechanism of multidrug resistance to chemotherapeutic agents. TBC1D9 functions as an energy-dependent membrane efflux pump for lipophilic compounds.

Alternate Names

KIAA0882TBC1 domain family member 9, MDR1, TBC1 domain family member 9A, TBC1 domain family, member 9, TBC1 domain family, member 9 (with GRAM domain), TBC1D9A

Entrez Gene IDs

23158 (Human)

Gene Symbol

TBC1D9

UniProt

NP_055945.1 (Human)

Additional TBC1D9 Products

Product Documents for TBC1D9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TBC1D9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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