Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1A5

Format

BSA Free

Concentration

1.0 mg/ml

View all TBCA Antibodies »

Product Specifications

Immunogen

Recombinant TBCA (1-108aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for TBCA Antibody (1A5) - BSA Free

Western Blot: TBCA Antibody (1A5)BSA Free [NBP1-30168]

Western Blot: TBCA Antibody (1A5)BSA Free [NBP1-30168]

Western Blot: TBCA Antibody (1A5) [NBP1-30168] - Cell lysates of LNCaP(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TBCA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Applications for TBCA Antibody (1A5) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TBCA

Tubulin folding cofactor A, also known as TBCA, is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest. Recombinant human TBCA protein was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CFA, chaperonin cofactor A, TCP1-chaperonin cofactor A, tubulin folding cofactor A, Tubulin-folding cofactor A, tubulin-specific chaperone a

Entrez Gene IDs

6902 (Human)

Gene Symbol

TBCA

UniProt

NP_004598 (Human)

Additional TBCA Products

Product Documents for TBCA Antibody (1A5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TBCA Antibody (1A5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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