TBCA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86288

Novus Biologicals, part of Bio-Techne
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NBP1-86288
NBP1-86288-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TBCA Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MRAEDGENYDIKKQAEILQESRMMIPDCQRRLEAAYLDLQRILENEKDLEEAEEY

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TBCA Antibody

Western Blot: TBCA Antibody [NBP1-86288]

Western Blot: TBCA Antibody [NBP1-86288]

Western Blot: TBCA Antibody [NBP1-86288] - Analysis in control (vector only transfected HEK293T lysate) and TBCA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: TBCA Antibody [NBP1-86288]

Immunocytochemistry/ Immunofluorescence: TBCA Antibody [NBP1-86288]

Immunocytochemistry/Immunofluorescence: TBCA Antibody [NBP1-86288] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & microtubules.
Immunohistochemistry-Paraffin: TBCA Antibody [NBP1-86288]

Immunohistochemistry-Paraffin: TBCA Antibody [NBP1-86288]

Immunohistochemistry-Paraffin: TBCA Antibody [NBP1-86288] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

Applications for TBCA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TBCA

Tubulin folding cofactor A, also known as TBCA, is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest. Recombinant human TBCA protein was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CFA, chaperonin cofactor A, TCP1-chaperonin cofactor A, tubulin folding cofactor A, Tubulin-folding cofactor A, tubulin-specific chaperone a

Gene Symbol

TBCA

Additional TBCA Products

Product Documents for TBCA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TBCA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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