Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TBLR1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide that maps to a region between residues 100 and 150 of human TBL1-related Protein 1 using the numbering given in SwissProt entry Q9BZK7 (GeneID 79718).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TBLR1 Antibody

Western Blot: TBLR1 Antibody [NB600-270]

Western Blot: TBLR1 Antibody [NB600-270]

Western Blot: TBLR1 Antibody [NB600-270] - Detection of Human and Mouse TBLR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TBLR1 antibody NB600-270 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: TBLR1 Antibody [NB600-270]

Immunohistochemistry-Paraffin: TBLR1 Antibody [NB600-270]

Immunohistochemistry-Paraffin: TBLR1 Antibody [NB600-270] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- TBLR1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: TBLR1 Antibody [NB600-270]

Immunoprecipitation: TBLR1 Antibody [NB600-270]

Immunoprecipitation: TBLR1 Antibody [NB600-270] - Detection of human TBLR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBLR1 antibody NB600-270 (lot NB600-270-2) used for IP at 6 ug per reaction. TBLR1 was also immunoprecipitated by a previous lot of this antibody (lot NB600-270-1). For blotting immunoprecipitated TBLR1, NB600-270 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for TBLR1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. In some cases, the antibody may be diluted further than indicated. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31428774).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TBLR1

The protein encoded by this gene has sequence similarity with members of the WD40 repeat-containing protein family. The WD40 group is a large family of proteins, which appear to have a regulatory function. It is believed that the WD40 repeats mediate protein-protein interactions and members of the family are involved in signal transduction, RNA processing, gene regulation, vesicular trafficking, cytoskeletal assembly and may play a role in the control of cytotypic differentiation.

Alternate Names

C21, DC42, F-box-like/WD repeat-containing protein TBL1XR1, FLJ12894, IRA1Transducin beta-like 1X-related protein 1, nuclear receptor co-repressor/HDAC3 complex subunit, Nuclear receptor corepressor/HDAC3 complex subunit TBLR1, TBLR1TBL1-related protein 1, transducin (beta)-like 1 X-linked receptor 1, transducin (beta)-like 1X-linked receptor 1

Entrez Gene IDs

79718 (Human)

Gene Symbol

TBL1XR1

UniProt

Q9BZK7 (Human)

Additional TBLR1 Products

Product Documents for TBLR1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TBLR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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