TBX3 Antibody (JE33-38)

Western Blot: TBX3 Antibody (JE33-38) [NBP3-32998]

Western Blot: TBX3 Antibody (JE33-38) [NBP3-32998] - Western blot analysis of TBX3 on JAR cell lysates with Rabbit anti-TBX3 antibody (NBP3-32998) at 1/500 dilution.

Lysates/proteins at 10 ug/Lane.

Predicted band size: 79 kDa
Observed band size: 79 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32998) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: TBX3 Antibody (JE33-38) [NBP3-32998]

Flow Cytometry: TBX3 Antibody (JE33-38) [NBP3-32998] - Flow cytometric analysis of HepG2 cells labeling TBX3.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32998, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).