Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI11H10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all TCAP Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TCAP (NP_003664) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

18.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TCAP Antibody (OTI11H10) - Azide and BSA Free

Western Blot: TCAP Antibody (OTI11H10)Azide and BSA Free [NBP2-74471]

Western Blot: TCAP Antibody (OTI11H10)Azide and BSA Free [NBP2-74471]

Western Blot: TCAP Antibody (OTI11H10) - Azide and BSA Free [NBP2-74471] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TCAP.
Immunohistochemistry: TCAP Antibody (OTI11H10) - Azide and BSA Free [NBP2-74471]

Immunohistochemistry: TCAP Antibody (OTI11H10) - Azide and BSA Free [NBP2-74471]

Immunohistochemistry: TCAP Antibody (OTI11H10) - Azide and BSA Free [NBP2-74471] - Analysis of Human adult heart tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for TCAP Antibody (OTI11H10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TCAP

Sarcomere assembly is regulated by the muscle protein titin. Titin is a giant elastic protein with kinase activity that extends half the length of a sarcomere. It serves as a scaffold to which myofibrils and other muscle related proteins are attached. This gene encodes a protein found in striated and cardiac muscle that binds to the titin Z1-Z2 domains and is a substrate of titin kinase, interactions thought to be critical to sarcomere assembly. Mutations in this gene are associated with limb-girdle muscular dystrophy type 2G. [provided by RefSeq]

Alternate Names

19 kDa sarcomeric protein, CMD1NTitin cap protein, LGMD2G, limb girdle muscular dystrophy 2G (autosomal recessive), T-cap, TELE, telethonin, titin-cap (telethonin)

Gene Symbol

TCAP

Additional TCAP Products

Product Documents for TCAP Antibody (OTI11H10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TCAP Antibody (OTI11H10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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