TCF-12/HTF4 Antibody (2E9)

Novus Biologicals, part of Bio-Techne | Catalog # H00006938-M01

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TCF-12/HTF4 Antibodies »

Product Specifications

Immunogen

TCF12 (NP_996919, 364 a.a. ~ 453 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VGSPSPLTGTSQWPRPGGQAPSSPSYENSLHSLKNRVEQQLHEHLQDAMSFLKDVCEQSRMEDRLDRLDDAIHVLRNHAVGPSTSLPAGH

Specificity

TCF12 - transcription factor 12 (HTF4, helix-loop-helix transcription factors 4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TCF-12/HTF4 Antibody (2E9)

Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]

Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - TCF12 monoclonal antibody (M01), clone 2E9 Analysis of TCF12 expression in Jurkat.

Immunocytochemistry/ Immunofluorescence: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]

Immunocytochemistry/Immunofluorescence: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - TCF12 ( Cat# H00006938-M01 ) on HeLa cell . [antibody concentration 10 ug/ml]

Immunohistochemistry-Paraffin: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]

Immunohistochemistry-Paraffin: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - Analysis of monoclonal antibody to TCF12 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.

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Applications for TCF-12/HTF4 Antibody (2E9)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against recominant protein and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TCF-12/HTF4

The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Long Name

Transcription Factor 12

Alternate Names

BHLHB20, HEB, HTF4, TCF12

Entrez Gene IDs

6938 (Human)

Gene Symbol

TCF12

OMIM

600480 (Human)

UniProt

NP_996919 (Human)

Additional TCF-12/HTF4 Products

Product Documents for TCF-12/HTF4 Antibody (2E9)

Product Datasheets

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COA

SDS

Product Specific Notices for TCF-12/HTF4 Antibody (2E9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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