Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4D6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all TCF-12/HTF4 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-250 of human TCF12 (NP_996919) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

75.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TCF-12/HTF4 Antibody (OTI4D6) - Azide and BSA Free

Western Blot: TCF-12/HTF4 Antibody (OTI4D6)Azide and BSA Free [NBP2-74473]

Western Blot: TCF-12/HTF4 Antibody (OTI4D6)Azide and BSA Free [NBP2-74473]

Western Blot: TCF-12/HTF4 Antibody (OTI4D6) - Azide and BSA Free [NBP2-74473] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TCF12.
Immunohistochemistry: TCF-12/HTF4 Antibody (OTI4D6) - Azide and BSA Free [NBP2-74473]

Immunohistochemistry: TCF-12/HTF4 Antibody (OTI4D6) - Azide and BSA Free [NBP2-74473]

Immunohistochemistry: TCF-12/HTF4 Antibody (OTI4D6) - Azide and BSA Free [NBP2-74473] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: TCF-12/HTF4 Antibody (OTI4D6) - Azide and BSA Free [NBP2-74473]

Immunohistochemistry: TCF-12/HTF4 Antibody (OTI4D6) - Azide and BSA Free [NBP2-74473]

Immunohistochemistry: TCF-12/HTF4 Antibody (OTI4D6) - Azide and BSA Free [NBP2-74473] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for TCF-12/HTF4 Antibody (OTI4D6) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TCF-12/HTF4

TCF12 is encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Long Name

Transcription Factor 12

Alternate Names

BHLHB20, HEB, HTF4, TCF12

Gene Symbol

TCF12

Additional TCF-12/HTF4 Products

Product Documents for TCF-12/HTF4 Antibody (OTI4D6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TCF-12/HTF4 Antibody (OTI4D6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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