Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TCF-12/HTF4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Transcription Factor 12 using the numbering given in entry NP_996919.1 (GeneID 6938).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCF-12/HTF4 Antibody

Western Blot: TCF-12/HTF4 Antibody [NB100-2337]

Western Blot: TCF-12/HTF4 Antibody [NB100-2337]

Western Blot: TCF-12/HTF4 Antibody [NB100-2337] - Detection of Human TCF12 on HeLa whole cell lysate using NB100-2337. TCF12 was also immunoprecipitated using rabbit anti-TCF12 antibody NB100-2338.
Immunohistochemistry: TCF-12/HTF4 Antibody [NB100-2337]

Immunohistochemistry: TCF-12/HTF4 Antibody [NB100-2337]

Immunohistochemistry: TCF-12/HTF4 Antibody [NB100-2337] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TCF12 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: TCF-12/HTF4 Antibody [NB100-2337]

Immunohistochemistry-Paraffin: TCF-12/HTF4 Antibody [NB100-2337]

Immunohistochemistry-Paraffin: TCF-12/HTF4 Antibody [NB100-2337] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-TCF12 used at a dilution of 1:250.

Applications for TCF-12/HTF4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TCF-12/HTF4

The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Long Name

Transcription Factor 12

Alternate Names

BHLHB20, HEB, HTF4, TCF12

Entrez Gene IDs

6938 (Human)

Gene Symbol

TCF12

UniProt

NP_996919.1 (Human)

Additional TCF-12/HTF4 Products

Product Documents for TCF-12/HTF4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TCF-12/HTF4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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