TCF-2/HNF-1 beta Antibody (CL0374) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43951

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-43951
Print Quote
Bulk Order
Forumulation
Catalog #
BSA Free
NBP2-30678
View Product
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0374

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all TCF-2/HNF-1 beta Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P35680, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TCF-2/HNF-1 beta Antibody (CL0374) - Azide and BSA Free

TCF-2/HNF-1 beta Antibody (CL0374) - Azide and BSA Free Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP3-43951]

Immunohistochemistry-Paraffin: TCF-2/HNF-1 beta Antibody [NBP3-43951]

Analysis in human kidney and tonsil tissues using NBP3-43951 antibody. Corresponding TCF-2/HNF-1 beta RNA-seq data are presented for the same tissues.
TCF-2/HNF-1 beta Antibody (CL0374) - Azide and BSA Free Western Blot: TCF-2/HNF-1 beta Antibody [NBP3-43951]

Western Blot: TCF-2/HNF-1 beta Antibody [NBP3-43951]

Lane 1: Marker [kDa]
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: TCF-2/HNF-1 beta Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
TCF-2/HNF-1 beta Antibody (CL0374) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: TCF-2/HNF-1 beta Antibody [NBP3-43951]

Immunocytochemistry/ Immunofluorescence: TCF-2/HNF-1 beta Antibody [NBP3-43951]

Staining in A549 cell line with Anti-TCF-2/HNF-1 beta monoclonal antibody) staining in green. Microtubule probes are visualized in red (where available).
View 6 more images

Applications for TCF-2/HNF-1 beta Antibody (CL0374) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCF-2/HNF-1 beta

TCF2 encodes transcription factor 2, a liver-specific factor of the homeobox-containing basic helix-turn-helix family. The TCF2 protein is believed to form heterodimers with another liver-specific member of this transcription factor family, TCF1; depending on the TCF2 isoform, the result may be to activate or inhibit transcription of target genes. Mutation of TCF2 that disrupts normal function has been identified as the cause of MODY5 (Maturity-Onset of Diabetes, Type 5). A third human transcript variant is believed to exist based on such a variant in the rat: however, to date such an mRNA species has not been isolated.

Long Name

Transcription Factor 2

Alternate Names

HNF-1 beta, HNF1B, HNF2, LF-B3, MODY5, TCF2, VHNF1

Gene Symbol

HNF1B

Additional TCF-2/HNF-1 beta Products

Product Documents for TCF-2/HNF-1 beta Antibody (CL0374) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TCF-2/HNF-1 beta Antibody (CL0374) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.