TCF-3/E2A Antibody (5G2)
Novus Biologicals, part of Bio-Techne | Catalog # H00006929-M01
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 5G2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
TCF3 (NP_003191, 545 a.a. ~ 654 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EREKERRVANNARERLRVRDINEAFKELGRMCQLHLNSEKPQTKLLILHQAVSVILNLEQQVRERNLNPKAACLKRREEEKVSGVVGDPQMVLSAPHPGLSEAHNPAGHM
Specificity
TCF3 - transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for TCF-3/E2A Antibody (5G2)
![Western Blot: TCF-3/E2A Antibody (5G2) [H00006929-M01]](https://resources.bio-techne.com/images/products/TCF-3-E2A-Antibody-5G2-Western-Blot-H00006929-M01-img0004.jpg "Western Blot: TCF-3/E2A Antibody (5G2) [H00006929-M01]")
Western Blot: TCF-3/E2A Antibody (5G2) [H00006929-M01]
Western Blot: TCF-3/E2A Antibody (5G2) [H00006929-M01] - TCF3 monoclonal antibody (M01), clone 5G2 Analysis of TCF3 expression in Hela S3 NE.
Sandwich ELISA: TCF-3/E2A Antibody (5G2) [H00006929-M01] - Detection limit for recombinant GST tagged TCF3 is approximately 0.03ng/ml as a capture antibody.
Applications for TCF-3/E2A Antibody (5G2)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TCF-3/E2A
Long Name
Transcription Factor 3/E2-alpha
Alternate Names
bHLHb21, E2A, ITF1, Kappa-E2-binding factor, Pan2, Tcfe2a, VDIR
Entrez Gene IDs
6929 (Human)
Gene Symbol
TCF3
OMIM
147141 (Human)
UniProt
Additional TCF-3/E2A Products
Product Documents for TCF-3/E2A Antibody (5G2)
Product Specific Notices for TCF-3/E2A Antibody (5G2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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