TCF-3/E2A Antibody (HL1954) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25713
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1954
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for TCF-3/E2A Antibody (HL1954) - Azide and BSA Free
Immunogen
Recombinant fragment of human TCF-3/E2A
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (82%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TCF-3/E2A Antibody (HL1954) - Azide and BSA Free
Western Blot: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] -
Western Blot: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF3 / E2A antibody [HL1954] (NBP3-25713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] -
Western Blot: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] - NCI-H929 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF3 / E2A antibody [HL1954] (NBP3-25713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] -
Immunocytochemistry/Immunofluorescence: TCF-3/E2A Antibody (HL1954) - Azide and BSA Free [NBP3-25713] - TCF3 / E2A antibody [HL1954] detects TCF3 / E2A protein by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TCF3 / E2A stained by TCF3 / E2A antibody [HL1954] (NBP3-25713) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.Applications for TCF-3/E2A Antibody (HL1954) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TCF-3/E2A
Long Name
Transcription Factor 3/E2-alpha
Alternate Names
bHLHb21, E2A, ITF1, Kappa-E2-binding factor, Pan2, Tcfe2a, VDIR
Gene Symbol
TCF3
Additional TCF-3/E2A Products
Product Documents for TCF-3/E2A Antibody (HL1954) - Azide and BSA Free
Product Specific Notices for TCF-3/E2A Antibody (HL1954) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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