TCF-3/E2A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20585
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human TCF3. The exact sequence is proprietary.
Reactivity Notes
Rat (83%)
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TCF-3/E2A Antibody
![Western Blot: TCF-3/E2A Antibody [NBP2-20585]](https://resources.bio-techne.com/images/products/TCF3-E2A-Antibody-Western-Blot-NBP2-20585-img0001.jpg "Western Blot: TCF-3/E2A Antibody [NBP2-20585]")
Western Blot: TCF-3/E2A Antibody [NBP2-20585]
Western Blot: TCF3/E2A Antibody [NBP2-20585] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:2000.![Immunoprecipitation: TCF-3/E2A Antibody [NBP2-20585]](https://resources.bio-techne.com/images/products/TCF-3-E2A-Antibody-Immunoprecipitation-NBP2-20585-img0004.jpg "Immunoprecipitation: TCF-3/E2A Antibody [NBP2-20585]")
Immunoprecipitation: TCF-3/E2A Antibody [NBP2-20585]
Immunoprecipitation: TCF-3/E2A Antibody [NBP2-20585] - Jurkat whole cell extract A. 35 ug Jurkat whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of TCF3 protein by 4 ug TCF3 antibody 7.5 % SDS-PAGE The immunoprecipitated TCF3 protein was detected by TCF3 antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Immunocytochemistry/ Immunofluorescence: TCF-3/E2A Antibody [NBP2-20585] -
Immunocytochemistry/ Immunofluorescence: TCF-3/E2A Antibody [NBP2-20585] - TCF-3/E2A antibody detects TCF3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TCF3 stained by TCF-3/E2A antibody (NBP2-20585) diluted at 1:600.Blue: Fluoroshield with DAPI .Applications for TCF-3/E2A Antibody
Application
Recommended Usage
Immunoprecipitation
1:100-1:500
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TCF-3/E2A
Long Name
Transcription Factor 3/E2-alpha
Alternate Names
bHLHb21, E2A, ITF1, Kappa-E2-binding factor, Pan2, Tcfe2a, VDIR
Gene Symbol
TCF3
UniProt
Additional TCF-3/E2A Products
Product Documents for TCF-3/E2A Antibody
Product Specific Notices for TCF-3/E2A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.