TCF4 Antibody (TCF4/2252) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14192
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2c Kappa Clone # TCF4/2252
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human TCF4 protein fragment (around aa 365-671) (exact sequence is proprietary) (Uniprot: P15884 )
Localization
Nucleus, Cytoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2c Kappa
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13789).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for TCF4 Antibody (TCF4/2252) - Azide and BSA Free
TCF4 Antibody (TCF4/2252)
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with TCF4 antibody (TCF4/2252) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
TCF4 Antibody (TCF4/2252)
Formalin-fixed, paraffin-embedded human breast carcinoma stained with TCF4 antibody (TCF4/2252) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for TCF4 Antibody (TCF4/2252) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
ELISA: For coating, order this antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: TCF4
Alternate Names
BHLHB19, bHLHb19E2-2, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, ITF-2, ITF2PTHS, MGC149724, SEF-2, SEF2-1, SEF2-1A, SEF2-1B, SEF2MGC149723, SL3-3 enhancer factor 2, TCF-4, transcription factor 4
Gene Symbol
TCF4
Additional TCF4 Products
Product Documents for TCF4 Antibody (TCF4/2252) - Azide and BSA Free
Product Specific Notices for TCF4 Antibody (TCF4/2252) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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