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TCF7/TCF1 Antibody (2E9)

Novus Biologicals, part of Bio-Techne | Catalog # H00006932-M02

Novus Biologicals, part of Bio-Techne
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H00006932-M02

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Cited:

Block/Neutralize, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

TCF7 (NP_003193, 287 a.a. ~ 384 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VIAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKRRSREKHQESTTGGKRNAFGTYPEKAAAPAPFLPMTVL

Specificity

TCF7 (2E9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TCF7/TCF1 Antibody (2E9)

Western Blot: TCF7/TCF1 Antibody (2E9) [H00006932-M02]

Western Blot: TCF7/TCF1 Antibody (2E9) [H00006932-M02]

Western Blot: TCF7/TCF1 Antibody (2E9) [H00006932-M02] - TCF7 monoclonal antibody (M02), clone 2E9 Analysis of TCF7 expression in Jurkat.
Immunohistochemistry-Paraffin: TCF7/TCF1 Antibody (2E9) [H00006932-M02]

Immunohistochemistry-Paraffin: TCF7/TCF1 Antibody (2E9) [H00006932-M02]

Immunohistochemistry-Paraffin: TCF7/TCF1 Antibody (2E9) [H00006932-M02] - Analysis of monoclonal antibody to TCF7 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Sandwich ELISA: TCF7/TCF1 Antibody (2E9) [H00006932-M02] - Detection limit for recombinant GST tagged TCF7 is approximately 0.03ng/ml as a capture antibody.

Applications for TCF7/TCF1 Antibody (2E9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TCF7/TCF1

The T cell specific transcription factor TCF7 activates genes involved in immune regulation and is a candidate locus for genetic susceptibility to type 1 diabetes.

Long Name

Transcription Factor 7

Alternate Names

TCF1

Entrez Gene IDs

6932 (Human)

Gene Symbol

TCF7

OMIM

189908 (Human)

Additional TCF7/TCF1 Products

Product Documents for TCF7/TCF1 Antibody (2E9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TCF7/TCF1 Antibody (2E9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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