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TCF7L1/TCF3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98907

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human TCF7L1/TCF3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for TCF7L1/TCF3 Antibody

Western Blot: TCF7L1/TCF3 Antibody [NBP2-98907]

Western Blot: TCF7L1/TCF3 Antibody [NBP2-98907]

Western Blot: TCF7L1/TCF3 Antibody [NBP2-98907] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:63 kDaObserved band size:63 kDa
Immunoprecipitation: TCF7L1/TCF3 Antibody [NBP2-98907]

Immunoprecipitation: TCF7L1/TCF3 Antibody [NBP2-98907]

Immunoprecipitation: TCF7L1/TCF3 Antibody [NBP2-98907] - Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-TCF7L1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TCF7L1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 63 kDaObserved band size: 95 kDa

Applications for TCF7L1/TCF3 Antibody

Application
Recommended Usage

Immunoprecipitation

4-8 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCF7L1/TCF3

TCF/LEF transcription factors, such as TCF7L1, are mediators of the Wnt (see MIM 164820) signaling pathway and areantagonized by the TGF-beta (TGFB1; MIM 190180) signaling pathway (Sagara and Katoh, 2000 (PubMed 11085512)).(suppliedby OMIM)

Long Name

Transcription Factor 7-like 1

Alternate Names

TCF3

Gene Symbol

TCF7L1

Additional TCF7L1/TCF3 Products

Product Documents for TCF7L1/TCF3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TCF7L1/TCF3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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