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TCFL5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49174

Novus Biologicals, part of Bio-Techne
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NBP2-49174
NBP2-49174-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for TCFL5 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KRNRSRMRQLDTNVERRALGEIQNVGEGATATQGAWQSSESSQANLGEQAQSGPQGGRSQRRERHNRMERDRRRRIRI

Reactivity Notes

Mouse (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCFL5 Antibody

Western Blot: TCFL5 Antibody [NBP2-49174]

Western Blot: TCFL5 Antibody [NBP2-49174]

Western Blot: TCFL5 Antibody [NBP2-49174] - Analysis in human cell line MOLT-4.
Immunocytochemistry/ Immunofluorescence: TCFL5 Antibody [NBP2-49174]

Immunocytochemistry/ Immunofluorescence: TCFL5 Antibody [NBP2-49174]

Immunocytochemistry/Immunofluorescence: TCFL5 Antibody [NBP2-49174] - Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TCFL5 Antibody [NBP2-49174]

Immunohistochemistry-Paraffin: TCFL5 Antibody [NBP2-49174]

Immunohistochemistry-Paraffin: TCFL5 Antibody [NBP2-49174] - Staining of human liver shows no positivity in hepatocytes as expected.

Applications for TCFL5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCFL5

TCFL5 is a new bHLH transcription factor that negatively regulates upstream transcription factor-dependent transcription.

Alternate Names

bHLHe82, CHA, Cha transcription factor, CHAMGC46135, E2BP1, E2BP-1HPV-16 E2 binding protein 1, Figlb, HPV-16 E2-binding protein 1, transcription factor-like 5 (basic helix-loop-helix), transcription factor-like 5 protein

Gene Symbol

TCFL5

Additional TCFL5 Products

Product Documents for TCFL5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TCFL5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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