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TCP1-eta Antibody (PK/16/8/a)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-66571

Novus Biologicals, part of Bio-Techne
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NB100-66571

Key Product Details

Species Reactivity

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # PK/16/8/a

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Made to CCT ETA

Reactivity Notes

Predicted cross-reactivities: Rabbit, Mouse, Human, Bovine

Specificity

NB100-66571 recognizes the eta polypeptide of the CCT chaperonin molecule complex. The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for TCP1-eta Antibody (PK/16/8/a)

Western Blot: TCP1-eta Antibody (PK/16/8/a)BSA Free [NB100-66571]

Western Blot: TCP1-eta Antibody (PK/16/8/a)BSA Free [NB100-66571]

Western Blot: TCP1-eta Antibody (PK/16/8/a) [NB100-66571] - Western blot analysis of HeLa whole cell lysate (1) and HeLa heat stressed whole cell lysate (2) probed with RAT ANTI CCT ETA followed by F(ab')2 RABBIT ANTI RAT IgG:HRP.

Applications for TCP1-eta Antibody (PK/16/8/a)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100

Western Blot

1:500
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. In WB detects a band of approximately 60kD in Hela cell lysates.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCP1-eta

This antibody recognizes the eta polypeptide of the CCT chaperonin molecule complex. The intact CCT complex is composed of eight polypeptides in a double ring structure. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP dependent manner. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumor suppressor protein. Alternate transcriptional splice variants encoding different isoforms have been found for this gene, but only two of them have been characterized to date.

Alternate Names

CCTETA, CCT-eta, CCTH, chaperonin containing TCP1, subunit 7 (eta), eta subunit, HIV-1 Nef interacting protein, HIV-1 Nef-interacting protein, MGC110985, Nip7-1, T-complex protein 1 subunit eta

Entrez Gene IDs

514355 (Human); 12468 (Mouse)

Gene Symbol

CCT7

UniProt

Additional TCP1-eta Products

Product Documents for TCP1-eta Antibody (PK/16/8/a)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TCP1-eta Antibody (PK/16/8/a)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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